LS-C2744

antibody from LSBio

Targeting: CDKN2A ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C2744

Provider

LSBio

Product name

Anti-CDKN2A / p16INK4a Antibody (aa54-75, clone 5-C3-1) LS-C2744

Antibody type

Monoclonal

Description

Immunoaffinity purified

Reactivity

Mouse

Host

Rat

Isotype

IgG

Antibody clone number

5-C3-1

Vial size

100µl

Storage

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of p19Arf using LS-C2744. Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (lane 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (lane 3), or LS-C2744 (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analysed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) f. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemical staining of sections from a wild type mouse testis taken at P11 were probed with LS-C2744 (green). Sections were stained with DAPI to reveal the position of cell nuclei (blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.