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antibody from Invitrogen Antibodies
Targeting: CDKN2A
ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [2]
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- Product number
- PA1-16662 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p14ARF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: BT549 or HeLa whole cell extracts, antigen standard for CDKN2A (transient overexpression lysate).
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of p14ARF uding PA1-16662. Samples: WCE (25 µg) from BT549, positive control and U205 negative control and transfected IMR90 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p14ARF in 25 µg WCE from BT549, positive control and U205 negative control and transfected IMR90 cells. Samples were incubated in p14ARF polyclonal antibody (Product # PA1-16662.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p14ARF in 25 µg WCE from BT549, positive control and U205 negative control and transfected IMR90 cells. Samples were incubated in p14ARF polyclonal antibody (Product # PA1-16662.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of p14ARF/p16INK4a was achieved by transfecting HeLa with p14ARF/p16INK4a specific siRNAs (Silencer® select Product # S216, S217). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the p14ARF/p16INK4a knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with p14ARF Polyclonal Antibody (Product # PA1-16662, 1:1000 ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to p14ARF/p16INK4a.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-p14ARF Polyclonal Antibody(Product # PA1-16662) and a 16kDa band corresponding to p14ARF/p16INK4a was observed in HeLa and DU 145, but not in OVCAR-3, U-2 OS and HEL 92.1.7. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), NIH:OVCAR-3 (Lane 2), DU 145 (Lane 3), U-2 OS (Lane 4), HEL 92.1.7 (Lane 5) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Both p14ARF and p16INK4a are products of the same gene CDKN2A, and are isoforms produced by alternate splicing.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of p14ARF in formalin-fixed paraffin-embedded section of human prostate carcinoma. Samples were incubated in p14ARF polyclonal antibody (Product # PA1-16662) using a dilution of 1:1,000. Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of p14ARF in formalin-fixed paraffin-embedded section of human prostate adenocarcinoma. Samples were incubated in p14ARF polyclonal antibody (Product # PA1-16662) using a dilution of 1:250.
