374988

antibody from Invitrogen Antibodies

Targeting: CLDN1 ILVASC, SEMP1

Provider product page for 374988

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Immunocytochemistry [1]
Other assay [2]

Submit

Product number: 374988 - Provider product page
Provider: Invitrogen Antibodies
Product name: Claudin 1 Monoclonal Antibody (2H10D10), Alexa Fluor™ 488
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: Reactivity has been confirmed with human Caco-2 and canine MDCK cell lysates, mouse kidney and intestinal lysates, frozen mouse intestine (jejunum) tissue, and rat kidney homogenates.
Reactivity: Human, Mouse, Rat, Canine
Host: Mouse
Conjugate: Green dye
Isotype: IgG
Antibody clone number: 2H10D10
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: 4° C, store in dark

CLDN1 protein structure - 374988 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 MicroRNA-155 (miR-155) inhibition results in stabilization of claudin-1 (CLDN1). A, Immunofluorescence (IF) staining of CLDN1 in oxygen-glucose deprivation ( OGD) /control inhibitor (OGD/IC), specific miR-155 inhibitor (OGD/I), and mimic ( OGD /M) cells. Arrows demonstrate cell membrane localization of CLDN1 in OGD /I cells. Imaging was performed with a Zeiss LSM 800 confocal microscope using tile scan and Z stack image acquisitions. Bar: 20 mum. B, Western blot analysis of CLDN1 protein expression in normoxic control (NC), OGD / IC , OGD /I, mimic control (OGD/ MC), and OGD /M samples. Graph: optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as the average relative density values. Mann-Whitney (Wilcoxon) test and Kruskal test were used to compare pairwise difference and overall difference, respectively. Overall differences were highly significant ( P =0.0095); the pairwise difference between NC and OGD /I, OGD / IC, and OGD /I, and between OGD / MC and OGD /M was also significant ( P =0.029). n=4 (for NC ) and n=6 (for other groups) independent experiments. C, CLDN1 ( CLDN 1 ) quantitative polymerase chain reaction was performed in all samples (4 samples per group) and expressed as the relative fold change compared with CLDN 1 expression in NC samples. Red bar demonstrates CLDN 1 expression in the positive control sample (a mix of cDNA s synthesized from total RNA from 18 different hum
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Claudin-1 (CLDN1) and zonula occludens protein-1 (ZO-1) colocalize and interact in oxygen-glucose deprivation (OGD)/specific miR-155 inhibitor (OGD/I) cells. Double immunofluorescence staining of OGD /I cells with anti-CLDN1 (A) and anti- ZO -1 (B) antibodies. C and D, Colocalization maps automatically generated using Fiji software demonstrate colocalization (yellow) and no colocalization (red pixels) of 2 channels in the cells (C); enlarged details in (D) depict the degree of colocalization on the cell borders. Bar: 10 mum. E, Orthogonal projection of human primary brain microvascular endothelial cells from the OGD /I group, coimmunostained with anti-CLDN1 (green) and anti- ZO -1 (red) antibodies. 4',6-Diamidino-2-phenylindole staining was used to visualize nuclei (blue). Imaging was performed with a Zeiss LSM 800 confocal microscope using tile scan and Z stack image acquisitions. F, Cell lysates from the OGD /I and OGD /control inhibitor (OGD/IC) groups (L- OGD /I and L- OGD / IC ) were immunoprecipitated with anti- ZO -1 antibody. Immunoprecipitates ( IP - OGD /I and IP - OGD / IC ) were probed with antibodies against ZO -1, CLDN1, actin, phosphotyrosine ( pT yr), and phosphoserine ( pS er). G through I, OGD -subjected cells were transfected with CLDN 1 cDNA -containing vector ( OGD + CLDN 1) or empty vector ( OGD + EV ). G, expression of ZO -1 and CLDN1 was detected in OGD + CLDN 1 (lane 1), normoxic control ( NC ; lane 2), and OGD + EV (lane 3) samples. Actin wa
Conjugate: Green dye