MA5-16351

antibody from Invitrogen Antibodies

Targeting: CLDN1 ILVASC, SEMP1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-16351

Provider

Invitrogen Antibodies

Product name

Claudin 1 Monoclonal Antibody (SP128)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is liver, hepatocellular carcinoma or ovarian carcinoma.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

SP128

Vial size

500 µL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Development of an Improved 3D in vitro Intestinal Model to Perform Permeability Studies of Paracellular Compounds.

Macedo MH, Martínez E, Barrias CC, Sarmento B
Frontiers in bioengineering and biotechnology 2020;8:524018

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CLDN1 Monoclonal Antibody (SP128) (Product # MA5-16351) and a 22 kDa band corresponding to CLDN1 was observed across cell lines tested and increased upon treatment. Membrane enriched extracts (30 µg lysate) of Caco-2 (Lane 1), Caco-2 treated with EGF (100ng/mL) and LiCl (50mM) simultaneously for 24 hr (Lane 2), Caco-2 treated with IL-beta (10ng/mL for 48 hr) (Lane 3), (25 ug lysate) of A-549 (Lane 4) and A-549 treated with TGF-beta (10ng/mL) and TNF-alpha (20ng/mL) simultaneously for 72 hr (Lane 5) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Claudin-1 using anti-Claudin-1 Monoclonal Antibody (Product # MA5-16351) in Hepatocellular Carcinoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 6 (A) Visualization of cell layer formation by EpCAM, confirming the presence of a cell layer in all models. (B) Assessment and comparison of cellular adherens junctions in different models at different time points using E -cadherin. (C) Assessment and comparison of the tight junctions' protein claudin-1 in the different models at different time points. EpCAM, E -cadherin, and claudin-1 were labeled with Alexa-Fluor 488 (green), and nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue).