701853

antibody from Invitrogen Antibodies

Targeting: BCL2L11 BIM, BimEL, BimL, BimS, BOD

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

701853

Provider

Invitrogen Antibodies

Product name

Bim Recombinant Rabbit Monoclonal Antibody (8H1L4)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This antibody is predicted to react with Monkey, Rabbit and Mouse.

Antibody clone number

8H1L4

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of Raji (Lane1), Daudi (Lane 2), Karpas (Lane 3), MCF-7 (Lane 4), THP-1 (lane 5), HEL 92.1.7 (Lane 6) and HeLa (Lane 7) treated with Paclitaxol (150 uM/ 24hours). The blots were probed with BIM Recombinant Rabbit Monoclonal Antibody (Product # 701853, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 30 kDa band corresponding to BIM was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of BIM was performed on MCF7 cells treated with Paclitaxel (150uM/24 hours) labeled with ABfinityª Anti-BIM Recombinant Rabbit Monoclonal Antibody (Product# 701853, 2-4 ug/ 1M cells) or with Rabbit isotype control and detected with Goat anti-Rabbit IgG (H+L) Superclonalª Secondary Antibody, (Alexa Fluor¨ 488 conjugate, Product # A27034, 0.4 ug/ml, 1:2500) as represented by the red and pink histograms respectively. The purple histogram represents unstained control cells and the green histogram represents no-primary-Antibody control. A representative of 10,000 cells were acquired and analyzed for each sample using an Attune¨ Acoustic Focusing Cytometer (4468770).