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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3160 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3160, RRID:AB_1570885
- Product name
- ACTB/ACTC1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ACTB/ACTC1.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400 uL
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references An active Src kinase-beta-actin association is linked to actin dynamics at the periphery of colon cancer cells.
Domain-specific chaperone-induced expansion is required for beta-actin folding: a comparison of beta-actin conformations upon interactions with GroEL and tail-less complex polypeptide 1 ring complex (TRiC).
Variable presentation of nemaline myopathy: novel mutation of alpha actin gene.
Avizienyte E, Keppler M, Sandilands E, Brunton VG, Winder SJ, Ng T, Frame MC
Experimental cell research 2007 Sep 10;313(15):3175-88
Experimental cell research 2007 Sep 10;313(15):3175-88
Domain-specific chaperone-induced expansion is required for beta-actin folding: a comparison of beta-actin conformations upon interactions with GroEL and tail-less complex polypeptide 1 ring complex (TRiC).
Villebeck L, Moparthi SB, Lindgren M, Hammarström P, Jonsson BH
Biochemistry 2007 Nov 6;46(44):12639-47
Biochemistry 2007 Nov 6;46(44):12639-47
Variable presentation of nemaline myopathy: novel mutation of alpha actin gene.
Bouldin AA, Parisi MA, Laing N, Patterson K, Gospe SM Jr
Muscle & nerve 2007 Feb;35(2):254-8
Muscle & nerve 2007 Feb;35(2):254-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ACTB/ACTC1 polyclonal antibody (Cat # PAB3160) in mouse testis tissue lysates (35 ug/lane). ACTB/ACTC1 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACTB/ACTC1 polyclonal antibody (Cat # PAB3160) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
