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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-16410 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta Actin Monoclonal Antibody (SP124)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP124
- Vial size
- 100 µL
- Concentration
- 0.015 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Continual naringin treatment benefits the recovery of traumatic brain injury in rats through reducing oxidative and inflammatory alterations.
Cui QJ, Wang LY, Wei ZX, Qu WS
Neurochemical research 2014 Jul;39(7):1254-62
Neurochemical research 2014 Jul;39(7):1254-62
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Human Placenta Cancer Tissue using anti-Actin-beta Monoclonal Antibody (Product # MA5-16410). The recommened dilution for this antibody in western blot applications is 1:25.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Actin-beta using anti-Actin-beta Monoclonal Antibody (Product # MA5-16410) in Placenta Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:200.
