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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-17397 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cullin 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references The E3 Ubiquitin-Protein Ligase Cullin 3 Regulates HIV-1 Transcription.
Langer S, Yin X, Diaz A, Portillo AJ, Gordon DE, Rogers UH, Marlett JM, Krogan NJ, Young JAT, Pache L, Chanda SK
Cells 2020 Sep 1;9(9)
Cells 2020 Sep 1;9(9)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CUL3 in extracts from Neuro2A and PC12 cell lysates using CUL3 polyclonal antibody (Product # PA5-17397).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Cullin 3 Polyclonal Antibody (Product # PA5-17397) and a 81 kDa band corresponding to CUL3 was observed across cell lines and tissues tested. (Fig. a) Whole cell extracts (30ug) of HeLa (Lane 1), PC-12 (Lane 2), Neuro-2a (Lane 3), L6 (Lane 4), U-2 OS (Lane 5), Hep G2 (Lane 6), MDA-MB-231 (Lane 7), MCF 10A (Lane 8) and T-47D (Lane 9), (Fig.b) tissue extracts (30ug) of Mouse Brain (Lane 1), Rat Liver (Lane 2), Mouse Liver (Lane 3), Rat Ovary (Lane 4) and Mouse Ovary (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
