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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-17615 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Syntaxin 1 Monoclonal Antibody (SP8)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-17615 targets Syntaxin-1A in ELISA, FACS, and WB applications and shows reactivity with Human, Mouse, Porcine, and Rat samples.
- Antibody clone number
- SP8
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Syntaxin 1 Monoclonal Antibody (SP8)(Product # MA5-17615) and a 30 kDa band corresponding to Syntaxin 1 was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2), Mouse Brain (Lane 3), Rat Brain (Lane 4) and Mouse Kidney (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Brain is a high expressing model for this protein and kidney, is a low expressing model.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded human cerebellum sections at 400x magnification with DAPI counterstain. Primary antibody used was a Syntaxin-1A monoclonal antibody (clone SP8) at a 1:500 dilution, followed by secondary antibody was a 488 fluorescent conjugated Goat anti-mouse Ig at a 1:500 dilution and Normal Goat Serum (blocking)
