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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- MAB1182 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Neprilysin/CD10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Neprilysin/CD10 in Western blots and direct ELISAs. In Western blots, less than 5% cross-reactivity with recombinant human ECE-2 and recombinant mouse Neprilysin is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P08473- Isotype
- IgG
- Antibody clone number
- 212504
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Neprilysin/CD10 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human Neprilysin/CD10 Monoclonal Antibody (Catalog # MAB1182, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
