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Other assayAntibody data
- Antibody Data
- Antigen structure
- References [26]
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- Validations
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- Product number
- MA5-12707 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclin D1 Monoclonal Antibody (DCS-11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-12707 targets Cyclin D1/Bcl-1 in FN applications and shows reactivity with Human, mouse, and Rat samples.
- Antibody clone number
- DCS-11
- Concentration
- 0.2 mg/mL
Submitted references CDK/cyclin dependencies define extreme cancer cell-cycle heterogeneity and collateral vulnerabilities.
p27 allosterically activates cyclin-dependent kinase 4 and antagonizes palbociclib inhibition.
CDK4 T172 phosphorylation is central in a CDK7-dependent bidirectional CDK4/CDK2 interplay mediated by p21 phosphorylation at the restriction point.
Aurora B kinase phosphorylates and instigates degradation of p53.
Differential regulation of cyclin-dependent kinase 4 (CDK4) and CDK6, evidence that CDK4 might not be activated by CDK7, and design of a CDK6 activating mutation.
Cyclic AMP inhibits the proliferation of thyroid carcinoma cell lines through regulation of CDK4 phosphorylation.
Orphan receptor small heterodimer partner suppresses tumorigenesis by modulating cyclin D1 expression and cellular proliferation.
Molecular characterization of spontaneous mesenchymal stem cell transformation.
Regulated activating Thr172 phosphorylation of cyclin-dependent kinase 4(CDK4): its relationship with cyclins and CDK "inhibitors".
p21Cip1 and p27Kip1 induce distinct cell cycle effects and differentiation programs in myeloid leukemia cells.
Cyclin D1 antagonizes BRCA1 repression of estrogen receptor alpha activity.
Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts.
Regulation of cyclin E protein levels through E2F-mediated inhibition of degradation.
Cyclin C/cdk3 promotes Rb-dependent G0 exit.
Cyclin C/cdk3 promotes Rb-dependent G0 exit.
Differential effects of p21(WAF1/CIP1) deficiency on MMTV-ras and MMTV-myc mammary tumor properties.
Metabolic stabilization of p27 in senescent fibroblasts correlates with reduced expression of the F-box protein Skp2.
Metabolic stabilization of p27 in senescent fibroblasts correlates with reduced expression of the F-box protein Skp2.
Reconstitution of cyclin D1-associated kinase activity drives terminally differentiated cells into the cell cycle.
Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes.
Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes.
Loss of p27Kip1 from cyclin E/cyclin-dependent kinase (CDK) 2 but not from cyclin D1/CDK4 complexes in cells transformed by polyamine biosynthetic enzymes.
Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice.
Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice.
Inhibition of basal and mitogen-stimulated pancreatic cancer cell growth by cyclin D1 antisense is associated with loss of tumorigenicity and potentiation of cytotoxicity to cisplatinum.
Angiotensin II activation of cyclin D1-dependent kinase activity.
Knudsen ES, Kumarasamy V, Nambiar R, Pearson JD, Vail P, Rosenheck H, Wang J, Eng K, Bremner R, Schramek D, Rubin SM, Welm AL, Witkiewicz AK
Cell reports 2022 Mar 1;38(9):110448
Cell reports 2022 Mar 1;38(9):110448
p27 allosterically activates cyclin-dependent kinase 4 and antagonizes palbociclib inhibition.
Guiley KZ, Stevenson JW, Lou K, Barkovich KJ, Kumarasamy V, Wijeratne TU, Bunch KL, Tripathi S, Knudsen ES, Witkiewicz AK, Shokat KM, Rubin SM
Science (New York, N.Y.) 2019 Dec 13;366(6471)
Science (New York, N.Y.) 2019 Dec 13;366(6471)
CDK4 T172 phosphorylation is central in a CDK7-dependent bidirectional CDK4/CDK2 interplay mediated by p21 phosphorylation at the restriction point.
Bisteau X, Paternot S, Colleoni B, Ecker K, Coulonval K, De Groote P, Declercq W, Hengst L, Roger PP
PLoS genetics 2013 May;9(5):e1003546
PLoS genetics 2013 May;9(5):e1003546
Aurora B kinase phosphorylates and instigates degradation of p53.
Gully CP, Velazquez-Torres G, Shin JH, Fuentes-Mattei E, Wang E, Carlock C, Chen J, Rothenberg D, Adams HP, Choi HH, Guma S, Phan L, Chou PC, Su CH, Zhang F, Chen JS, Yang TY, Yeung SC, Lee MH
Proceedings of the National Academy of Sciences of the United States of America 2012 Jun 12;109(24):E1513-22
Proceedings of the National Academy of Sciences of the United States of America 2012 Jun 12;109(24):E1513-22
Differential regulation of cyclin-dependent kinase 4 (CDK4) and CDK6, evidence that CDK4 might not be activated by CDK7, and design of a CDK6 activating mutation.
Bockstaele L, Bisteau X, Paternot S, Roger PP
Molecular and cellular biology 2009 Aug;29(15):4188-200
Molecular and cellular biology 2009 Aug;29(15):4188-200
Cyclic AMP inhibits the proliferation of thyroid carcinoma cell lines through regulation of CDK4 phosphorylation.
Rocha AS, Paternot S, Coulonval K, Dumont JE, Soares P, Roger PP
Molecular biology of the cell 2008 Nov;19(11):4814-25
Molecular biology of the cell 2008 Nov;19(11):4814-25
Orphan receptor small heterodimer partner suppresses tumorigenesis by modulating cyclin D1 expression and cellular proliferation.
Zhang Y, Xu P, Park K, Choi Y, Moore DD, Wang L
Hepatology (Baltimore, Md.) 2008 Jul;48(1):289-98
Hepatology (Baltimore, Md.) 2008 Jul;48(1):289-98
Molecular characterization of spontaneous mesenchymal stem cell transformation.
Rubio D, Garcia S, Paz MF, De la Cueva T, Lopez-Fernandez LA, Lloyd AC, Garcia-Castro J, Bernad A
PloS one 2008 Jan 2;3(1):e1398
PloS one 2008 Jan 2;3(1):e1398
Regulated activating Thr172 phosphorylation of cyclin-dependent kinase 4(CDK4): its relationship with cyclins and CDK "inhibitors".
Bockstaele L, Kooken H, Libert F, Paternot S, Dumont JE, de Launoit Y, Roger PP, Coulonval K
Molecular and cellular biology 2006 Jul;26(13):5070-85
Molecular and cellular biology 2006 Jul;26(13):5070-85
p21Cip1 and p27Kip1 induce distinct cell cycle effects and differentiation programs in myeloid leukemia cells.
Muñoz-Alonso MJ, Acosta JC, Richard C, Delgado MD, Sedivy J, León J
The Journal of biological chemistry 2005 May 6;280(18):18120-9
The Journal of biological chemistry 2005 May 6;280(18):18120-9
Cyclin D1 antagonizes BRCA1 repression of estrogen receptor alpha activity.
Wang C, Fan S, Li Z, Fu M, Rao M, Ma Y, Lisanti MP, Albanese C, Katzenellenbogen BS, Kushner PJ, Weber B, Rosen EM, Pestell RG
Cancer research 2005 Aug 1;65(15):6557-67
Cancer research 2005 Aug 1;65(15):6557-67
Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts.
Hutter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E
The Biochemical journal 2004 Jun 15;380(Pt 3):919-28
The Biochemical journal 2004 Jun 15;380(Pt 3):919-28
Regulation of cyclin E protein levels through E2F-mediated inhibition of degradation.
Pajalunga D, Crescenzi M
Cell cycle (Georgetown, Tex.) 2004 Dec;3(12):1572-8
Cell cycle (Georgetown, Tex.) 2004 Dec;3(12):1572-8
Cyclin C/cdk3 promotes Rb-dependent G0 exit.
Ren S, Rollins BJ
Cell 2004 Apr 16;117(2):239-51
Cell 2004 Apr 16;117(2):239-51
Cyclin C/cdk3 promotes Rb-dependent G0 exit.
Ren S, Rollins BJ
Cell 2004 Apr 16;117(2):239-51
Cell 2004 Apr 16;117(2):239-51
Differential effects of p21(WAF1/CIP1) deficiency on MMTV-ras and MMTV-myc mammary tumor properties.
Bearss DJ, Lee RJ, Troyer DA, Pestell RG, Windle JJ
Cancer research 2002 Apr 1;62(7):2077-84
Cancer research 2002 Apr 1;62(7):2077-84
Metabolic stabilization of p27 in senescent fibroblasts correlates with reduced expression of the F-box protein Skp2.
Wagner M, Hampel B, Hütter E, Pfister G, Krek W, Zwerschke W, Jansen-Dürr P
Experimental gerontology 2001 Dec;37(1):41-55
Experimental gerontology 2001 Dec;37(1):41-55
Metabolic stabilization of p27 in senescent fibroblasts correlates with reduced expression of the F-box protein Skp2.
Wagner M, Hampel B, Hütter E, Pfister G, Krek W, Zwerschke W, Jansen-Dürr P
Experimental gerontology 2001 Dec;37(1):41-55
Experimental gerontology 2001 Dec;37(1):41-55
Reconstitution of cyclin D1-associated kinase activity drives terminally differentiated cells into the cell cycle.
Latella L, Sacco A, Pajalunga D, Tiainen M, Macera D, D'Angelo M, Felici A, Sacchi A, Crescenzi M
Molecular and cellular biology 2001 Aug;21(16):5631-43
Molecular and cellular biology 2001 Aug;21(16):5631-43
Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes.
Hennigan RF, Stambrook PJ
Molecular biology of the cell 2001 Aug;12(8):2352-63
Molecular biology of the cell 2001 Aug;12(8):2352-63
Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes.
Hennigan RF, Stambrook PJ
Molecular biology of the cell 2001 Aug;12(8):2352-63
Molecular biology of the cell 2001 Aug;12(8):2352-63
Loss of p27Kip1 from cyclin E/cyclin-dependent kinase (CDK) 2 but not from cyclin D1/CDK4 complexes in cells transformed by polyamine biosynthetic enzymes.
Ravanko K, Järvinen K, Paasinen-Sohns A, Hölttä E
Cancer research 2000 Sep 15;60(18):5244-53
Cancer research 2000 Sep 15;60(18):5244-53
Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice.
Tong W, Pollard JW
Molecular and cellular biology 1999 Mar;19(3):2251-64
Molecular and cellular biology 1999 Mar;19(3):2251-64
Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice.
Tong W, Pollard JW
Molecular and cellular biology 1999 Mar;19(3):2251-64
Molecular and cellular biology 1999 Mar;19(3):2251-64
Inhibition of basal and mitogen-stimulated pancreatic cancer cell growth by cyclin D1 antisense is associated with loss of tumorigenicity and potentiation of cytotoxicity to cisplatinum.
Kornmann M, Arber N, Korc M
The Journal of clinical investigation 1998 Jan 15;101(2):344-52
The Journal of clinical investigation 1998 Jan 15;101(2):344-52
Angiotensin II activation of cyclin D1-dependent kinase activity.
Watanabe G, Lee RJ, Albanese C, Rainey WE, Batlle D, Pestell RG
The Journal of biological chemistry 1996 Sep 13;271(37):22570-7
The Journal of biological chemistry 1996 Sep 13;271(37):22570-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Differential CDK/cyclin requirements in RB-proficient models (A) Color bar shows clusters organized by IC 50 of palbociclib with the relative sensitivity to the indicated CDK and cyclin genes depicted in the heatmap for breast and ovarian cancer cell lines. Boxplots summarize the IC 50 from the indicated clusters (****p < 0.0001 as determined by t test). (B) Oncoprint depicts genetic features of breast cancer cell lines relative to the clustering. Specific cell lines used functionally are summarized in the box. (C) Live cell imaging was used to explore the impact of CCNE1 knockdown in the absence and presence of palbociclib in the MB157 cell line. Error bars indicate means and SDs from triplicate, and experiments were done at 2 independent times. Biochemical characterization of the effect of CCNE1 knockdown in the MB157 cell model. (D-F) Live cell imaging to monitor the growth of MB157 in the absence and presence of palbociclib following CDK4/6 knockdown (D). The means and SDs are shown. The experiment was performed in triplicate. (E) Live cell imaging on HCC1806 cells to determine the effect of CCND1 knockdown in the presence or absence of palbociclib. Means and SDs were calculated and the experiments were done in triplicate at 2 independent times. (F) Complex formation between cyclin D1 and other CDKs and p27 KIP1 in HCC1806 and MCF7 cell lines was determined by co-immunoprecipitation. (G) Immunoprecipitated cyclin D1 from MCF7 and HCC1806 cells was used in kinase
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. Differential requirements of RB-deficient cells on cyclin E and p130 (A and B) Live cell imaging to track the division of MB436 and MB468 cells treated with palbociclib and/or the knockdown of CDK4 and CDK6 (A). Means and SDs are shown and calculated from triplicate. (B) Co-immunoprecipitation of cyclin D1 showing the failure to assemble complexes with the indicated proteins. (C) Live cell imaging to demonstrate the differential effect of CCNE1 knockdown in MB436 and MB468 cell lines in the presence or absence of palbociclib. Error bars indicate means and SDs. Experiments were done in triplicate and repeated at 2 independent times. (D) Biochemical characterization of the differential effect of CCNE1 knockdown in MB436 and MB468 cell models. (E) Biochemical analysis of the impact of CCNE1 and RBL2 knockdown on cell-cycle proteins. (F) Live-cell imaging to track the localization of CDK2 sensor in the nucleus and cytoplasm at the indicated time points in MB436 and MB468 cell lines following CCNE1 knockdown (nuclear localization indicates low kinase activity and cytoplasmic localization indicates high kinase activity; scale bar, 50 um). (G) MB157 parental model and model with RB deleted were used to evaluate the effect of CCNE1 knockdown on the indicated proteins by immunoblotting. (H) Live-cell imaging and BrdU incorporation assay were performed in MB157 cell line to delineate the effect of RB deletion on the sensitivity to CDK2 or CCNE1 knockdown. Column represents me
