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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-37487 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Cyclin D1 (Thr286) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is MCF-7 cells.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from MCF-7 tissue using Cyclin D1 (pThr286) polyclonal antibody (Product # PA5-37487) (left) or the same antibody preincubated with a blocking peptide (right).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-Cyclin D1 (Thr286) in paraffin-embedded human small intestinal carcinoma tissue. 1) Phospho-Cyclin D1 (Thr286) Polyclonal Antibody (Product # PA5-37487) was diluted at 1:200 (4° overnight). 2) Sodium citrate pH 6.0 was used for antigen retrieval (>98°C,20 min). 3) Secondary antibody was diluted at 1:233.
