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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-29241 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSC70 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, Raw264.7, mouse brain.
- Concentration
- 0.79 mg/mL
Submitted references Mitochondrial proteome remodeling in ischemic heart failure.
Liu T, Chen L, Kim E, Tran D, Phinney BS, Knowlton AA
Life sciences 2014 Apr 17;101(1-2):27-36
Life sciences 2014 Apr 17;101(1-2):27-36
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HSC70 Polyclonal Antibody (Product # PA5-29241). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 7.5% SDS PAGE. HSC70 Polyclonal Antibody (Product # PA5-29241) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HSC70 Polyclonal Antibody (Product # PA5-29241). Sample (20 µg of whole cell lysate). Lane A: Raw264.7. HSC70 Polyclonal Antibody (Product # PA5-29241) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HSC70 Polyclonal Antibody (Product # PA5-29241). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299. Lane C: HeLa. Lane D: HepG2. 7.5% SDS PAGE. HSC70 Polyclonal Antibody (Product # PA5-29241) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using HSC70 (Product # PA5-29241) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
