NBP1-77944

antibody from Novus Biologicals

Targeting: HSP90AA1 FLJ31884, Hsp89, Hsp90, HSP90N, HSPC1, HSPCA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-77944

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-77944, RRID:AB_11022924

Product name

Rabbit Polyclonal HSP90 alpha Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This affinity purified antibody is directed against human Hsp90 protein acetylated at K294.

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.96 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HSP90 alpha [ac Lys294] Antibody [NBP1-77944] - Shows detection of a band at ~90 kDa corresponding to Hsp90 in an SkBr3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor). Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA). Immunoprecipitation with anti-Hsp90 was performed prior to western blotting with anti-Hsp90 acetyl K294.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: HSP90 alpha [ac Lys294] Antibody [NBP1-77944] - Used at 20 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.