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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF575 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Chicken Polyclonal E-Selectin/CD62E Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified from egg yolks. Detects mouse E-Selectin/CD62E in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human E-Selectin and recombinant rat E-Selectin is observed.
- Reactivity
- Mouse
- Host
- Chicken/Avian
- Conjugate
- Unconjugated
- Isotype
- IgY
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation.
The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
Plasminogen activator inhibitor-1 regulates neutrophil influx during acute pyelonephritis.
Selective inhibition of proprotein convertases represses the metastatic potential of human colorectal tumor cells.
Mathias D, Mitchel RE, Barclay M, Wyatt H, Bugden M, Priest ND, Whitman SC, Scholz M, Hildebrandt G, Kamprad M, Glasow A
PloS one 2015;10(3):e0119661
PloS one 2015;10(3):e0119661
Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation.
Leeper NJ, Tedesco MM, Kojima Y, Schultz GM, Kundu RK, Ashley EA, Tsao PS, Dalman RL, Quertermous T
American journal of physiology. Heart and circulatory physiology 2009 May;296(5):H1329-35
American journal of physiology. Heart and circulatory physiology 2009 May;296(5):H1329-35
The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
Kato N, Yuzawa Y, Kosugi T, Hobo A, Sato W, Miwa Y, Sakamoto K, Matsuo S, Kadomatsu K
Journal of the American Society of Nephrology : JASN 2009 Jul;20(7):1565-76
Journal of the American Society of Nephrology : JASN 2009 Jul;20(7):1565-76
Plasminogen activator inhibitor-1 regulates neutrophil influx during acute pyelonephritis.
Roelofs JJ, Teske GJ, Bonta PI, de Vries CJ, Meijers JC, Weening JJ, van der Poll T, Florquin S
Kidney international 2009 Jan;75(1):52-9
Kidney international 2009 Jan;75(1):52-9
Selective inhibition of proprotein convertases represses the metastatic potential of human colorectal tumor cells.
Scamuffa N, Siegfried G, Bontemps Y, Ma L, Basak A, Cherel G, Calvo F, Seidah NG, Khatib AM
The Journal of clinical investigation 2008 Jan;118(1):352-63
The Journal of clinical investigation 2008 Jan;118(1):352-63
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- E-Selectin/CD62E in Mouse Thymus. E-Selectin/CD62E was detected in perfusion fixed frozen sections of mouse thymus using Mouse E-Selectin/CD62E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF575) at 1.7 µg/mL overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
