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- Product number
- PA5-29946 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- E-selectin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, Molt-4, Raji.
- Concentration
- 1 mg/mL
Submitted references The increased adhesion of tumor cells to endothelial cells after irradiation can be reduced by FAK-inhibition.
Nguemgo Kouam P, Bühler H, Hero T, Adamietz IA
Radiation oncology (London, England) 2019 Feb 4;14(1):25
Radiation oncology (London, England) 2019 Feb 4;14(1):25
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using E-selectin Polyclonal Antibody (Product # PA5-29946). Sample (30 µg of whole cell lysate). Lane A: H1299 . 7.5% SDS PAGE. E-selectin Polyclonal Antibody (Product # PA5-29946) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-E-selectin Polyclonal Antibody (Product # PA5-29946) and a 115 kDa band corresponding to E-selectin was observed in the cell line tested upon TNF alpha and IL-1 beta treatment. Membrane enriched extracts (30 µg lysate) of HUVEC (Lane 1), HUVEC treated with TNF alpha (10 pg/ml for 6 Hours) (Lane 2) and HUVEC treated with IL-1 beta (10 pg/ml for 6 Hours) (Lane 3) were electrophoresed using Novex® NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Influence of irradiation on the expression of adhesion proteins. Western Blot analysis with samples from primary HUVEC ( a, e ), breast cancer cells (MDA-MB-231 ( b, f ) and glioblastoma cells (U-373 MG ( c, g ) and U-87 MG ( d, h )). Above : a - d ) The sample was isolated 4 h after irradiation with 4 Gy. What is shown is the average value normalized to non-irradiated (0 Gy) values. Data represent means +- standard deviation (SD) from at least three separate experiments (n >= 3). * p < 0.05; ** p < 0.01; *** p < 0.001 (Student's t test). Below : e - h ) Examples of one single experiment each. Protein expression in Western blots 4, 12 and 24 h after irradiation with 4 Gy or 8 Gy
