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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Immunohistochemistry [2]
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- Product number
- BBA18 - Provider product page
- Provider
- R&D Systems
- Product name
- Human E-Selectin/CD62E Antibody
- Antibody type
- Polyclonal
- Description
- N/A. This antibody has been screened using CHO cells transfected with cDNAs for E-Selectin, P-Selectin, L-Selectin, ICAM-1 and VCAM-1. This antibody has shown to be only reactive with E-Selectin.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 500 uL
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Outer membrane vesicles derived from Escherichia coli up-regulate expression of endothelial cell adhesion molecules in vitro and in vivo.
ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
Endothelial adhesion molecule expression is unaltered in the peripheral nerve from patients with AIDS and distal sensory polyneuropathy.
TNFalpha increases the inflammatory response to vascular balloon injury without accelerating neointimal formation.
Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi.
E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells.
E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells.
Kim JH, Yoon YJ, Lee J, Choi EJ, Yi N, Park KS, Park J, Lötvall J, Kim YK, Gho YS
PloS one 2013;8(3):e59276
PloS one 2013;8(3):e59276
ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
Wilhelmsen K, Mesa KR, Lucero J, Xu F, Hellman J
The Journal of biological chemistry 2012 Aug 3;287(32):26478-94
The Journal of biological chemistry 2012 Aug 3;287(32):26478-94
Endothelial adhesion molecule expression is unaltered in the peripheral nerve from patients with AIDS and distal sensory polyneuropathy.
Fenzi F, Rossi F, Rava M, Cavallaro T, Ferrari S, Rizzuto N
Journal of neuroimmunology 2006 Sep;178(1-2):111-6
Journal of neuroimmunology 2006 Sep;178(1-2):111-6
TNFalpha increases the inflammatory response to vascular balloon injury without accelerating neointimal formation.
Miller AM, McPhaden AR, Preston A, Wadsworth RM, Wainwright CL
Atherosclerosis 2005 Mar;179(1):51-9
Atherosclerosis 2005 Mar;179(1):51-9
Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi.
Humphreys TL, Baldridge LA, Billings SD, Campbell JJ, Spinola SM
Infection and immunity 2005 Jul;73(7):3896-902
Infection and immunity 2005 Jul;73(7):3896-902
E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells.
Tilghman RW, Hoover RL
FEBS letters 2002 Aug 14;525(1-3):83-7
FEBS letters 2002 Aug 14;525(1-3):83-7
E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells.
Tilghman RW, Hoover RL
FEBS letters 2002 Aug 14;525(1-3):83-7
FEBS letters 2002 Aug 14;525(1-3):83-7
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- E-Selectin/CD62E in Human Kidney Cancer Tissue. E-Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human E-Selectin/ CD62E Polyclonal Antibody (Catalog # BBA18) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- E-Selectin/CD62E in LPS treated Human Kidney Tissue. E-Selectin/CD62E was detected in acetone fixed cryosections of human kidney tissue using Goat Anti-Human E-Selectin/CD62E Polyclonal Antibody (Catalog # BBA18) overnight at 4 ° C. Tissues were stained with rabbit anti-goat secondary antibody and HRP polymer-conjugated anti-rabbit IgG followed by AEC+Substrate Chromogen (red) followed by counterstaining with hematoxylin (blue). Before incubation with the primary antibody, tissues were treated ex vivo with LPS and also treated to block endogenous peroxidase activity. The lower panel shows an absence of labeling when primary antibody is omitted. Experiments were carried out and images were provided by Dr. Grietje Molema, University of Groningen, The Netherlands.
