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antibody from Invitrogen Antibodies
Targeting: SELL
CD62L, hLHRc, LAM-1, LAM1, Leu-8, LNHR, LSEL, Lyam-1, LYAM1, PLNHR
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [2]
- Other assay [1]
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- Product number
- 63-0629-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), Super Bright™ 600, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.
- Antibody clone number
- DREG-56 (DREG56)
- Concentration
- 5 µL/Test
Submitted references Induction of memory-like CD8+ T cells and CD4+ T cells from human naive T cells in culture.
Tokumoto Y, Araki Y, Narizuka Y, Mizuno Y, Ohshima S, Mimura T
Clinical and experimental immunology 2022 Jan 28;207(1):95-103
Clinical and experimental immunology 2022 Jan 28;207(1):95-103
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with CD4 Monoclonal Antibody, FITC (Product # 11-0048) and Mouse IgG1 kappa Isotype Control, Super Bright 600 (Product # 63-4714) (left) or CD62L Monoclonal Antibody, Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with CD4 Monoclonal Antibody, FITC (Product # 11-0048) and Mouse IgG1 kappa Isotype Control, Super Bright 600 (Product # 63-4714) (left) or CD62L Monoclonal Antibody, Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1. Activated CD8 + T cells in either normal or hypoxic culture. Naive CD8 + T cells derived from a healthy donor were cultured in human T-activator CD3/CD28 and IL-2 containing medium for 8 days. ( A ) in 20% O 2 condition and ( B ) in 1% O 2 condition. The 7-AAD-negative cells in the area gated as P1 in the FSC/SSC panel were considered as living cells. The expression pattern of CD45RA, CD62L, and CD127 of living cells were analyzed by FACS. The numbers on the FACS-plot panel mean the frequencies (%) of population of cells. We repeated this experiment four times.
