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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- NBP2-47972-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CEACAM/CD66 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. This antibody recognizes proteins of 80-200kDa, identified as different members of CEACAM family. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEACAM is not found in benign glands, stroma, or malignant prostatic cells
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CEACAM/CD66 Antibody (COL-1) - Azide and BSA Free [NBP2-47972] - Human Stomach lysate using CEA Mouse Monoclonal Antibody (COL-1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CEACAM/CD66 Antibody (COL-1) - Azide and BSA Free [NBP2-47972] - Human Colon Carcinoma stained with CEA Mouse Monoclonal Antibody (COL-1).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CEACAM/CD66 Antibody (COL-1) - Azide and BSA Free [NBP2-47972] - Formalin-fixed, paraffin-embedded human colon cancer stained with CEA COL-1 antibody at 1:200 using peroxidase-conjugate and DAB chromogen.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CEACAM/CD66 Antibody (COL-1) - Azide and BSA Free [NBP2-47972] - Formalin-fixed, paraffin-embedded normal human colon stained with CEA COL-1 antibody at 1:200 using peroxidase-conjugate and DAB chromogen.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): CEACAM/CD66 Antibody (COL-1) - Azide and BSA Free [NBP2-47972] - An intracellular stain was performed on A549 cells with CEACAM/CD66 Antibody (COL-1) NBP2-33306PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the Azide and BSA Free format of this antibody.
