AF2244

antibody from R&D Systems

Targeting: CEACAM1 BGP, BGP1, CD66a

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2244

Provider

R&D Systems

Product name

Human CEACAM-1/CD66a Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human CEACAM-1/CD66a in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P13688


Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Prognostic Impact of CEACAM1 in Node-Negative Ovarian Cancer Patients.

Oliveira-Ferrer L, Goswami R, Galatenko V, Ding Y, Eylmann K, Legler K, Kürti S, Schmalfeldt B, Milde-Langosch K
Disease markers 2018;2018:6714287

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Cytomegalovirus impairs cytotrophoblast-induced lymphangiogenesis and vascular remodeling in an in vivo human placentation model.

Tabata T, Petitt M, Fang-Hoover J, Rivera J, Nozawa N, Shiboski S, Inoue N, Pereira L
The American journal of pathology 2012 Nov;181(5):1540-59

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Glycosylation-dependent interactions of C-type lectin DC-SIGN with colorectal tumor-associated Lewis glycans impair the function and differentiation of monocyte-derived dendritic cells.

Nonaka M, Ma BY, Murai R, Nakamura N, Baba M, Kawasaki N, Hodohara K, Asano S, Kawasaki T
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3347-56

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Glycosylation-dependent interactions of C-type lectin DC-SIGN with colorectal tumor-associated Lewis glycans impair the function and differentiation of monocyte-derived dendritic cells.

Nonaka M, Ma BY, Murai R, Nakamura N, Baba M, Kawasaki N, Hodohara K, Asano S, Kawasaki T
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3347-56

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CEA-related cell adhesion molecule-1 is involved in angiogenic switch in prostate cancer.

Tilki D, Irmak S, Oliveira-Ferrer L, Hauschild J, Miethe K, Atakaya H, Hammerer P, Friedrich MG, Schuch G, Galalae R, Stief CG, Kilic E, Huland H, Ergun S
Oncogene 2006 Aug 17;25(36):4965-74

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human CEACAM-1/CD66a by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CEACAM-1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2244) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CEACAM-1/CD66a at approximately 100-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

CEACAM-1/CD66a in Human Colon Tissue. CEACAM-1/CD66a was detected in immersion fixed paraffin-embedded sections of human colon tissue using Goat Anti-Human CEACAM-1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2244) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.