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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [19]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB110-89062 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-89062, RRID:AB_1217495
- Product name
- Mouse Monoclonal Osteopontin/OPN Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Rat, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Plaque calcification is driven by different mechanisms of mineralization associated with specific cardiovascular risk factors.
Is there an optimal initial amount of activation for midpalatal suture expansion? : A histomorphometric and immunohistochemical study in a rabbit model.
Local application of osteoprotegerin-chitosan gel in critical-sized defects in a rabbit model.
Degradation of elastic fiber and elevated elastase expression in long head of biceps tendinopathy.
Novel device for application of continuous mechanical tensile strain to mammalian cells.
In vitro evaluation of osteoprotegerin in chitosan for potential bone defect applications.
Osteopontin, E-cadherin, and β-catenin expression as prognostic biomarkers in patients with radically resected gastric cancer.
A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration.
Intratracheal instillation of single-wall carbon nanotubes in the rat lung induces time-dependent changes in gene expression.
Osteopontin expression in biopsies of calciphylaxis.
Nanoparticle biphasic calcium phosphate loading on gelatin-pectin scaffold for improved bone regeneration.
Spinal but not cortical microglia acquire an atypical phenotype with high VEGF, galectin-3 and osteopontin, and blunted inflammatory responses in ALS rats.
Expression of osteogenic molecules in the caudate nucleus and gray matter and their potential relevance for Basal Ganglia calcification in hypoparathyroidism.
Magnesium-based bone implants: immunohistochemical analysis of peri-implant osteogenesis by evaluation of osteopontin and osteocalcin expression.
A combination of biphasic calcium phosphate scaffold with hyaluronic acid-gelatin hydrogel as a new tool for bone regeneration.
Expression of chondro-osteogenic BMPs in clinical samples of patellar tendinopathy.
Cellular retinol-binding protein 1 (CRBP-1) regulates osteogenenesis and adipogenesis of mesenchymal stem cells through inhibiting RXRα-induced β-catenin degradation.
Mapping of functional epitopes of osteopontin by monoclonal antibodies raised against defined internal sequences.
Mapping of functional epitopes of osteopontin by monoclonal antibodies raised against defined internal sequences.
Scimeca M, Anemona L, Granaglia A, Bonfiglio R, Urbano N, Toschi N, Santeusanio G, Schiaroli S, Mauriello S, Tancredi V, Schillaci O, Bonanno E, Mauriello A
Nutrition, metabolism, and cardiovascular diseases : NMCD 2019 Dec;29(12):1330-1336
Nutrition, metabolism, and cardiovascular diseases : NMCD 2019 Dec;29(12):1330-1336
Is there an optimal initial amount of activation for midpalatal suture expansion? : A histomorphometric and immunohistochemical study in a rabbit model.
Alyessary AS, Yap AU, Othman SA, Rahman MT, Al-Namnam NM, Radzi Z
Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie 2018 May;79(3):169-179
Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie 2018 May;79(3):169-179
Local application of osteoprotegerin-chitosan gel in critical-sized defects in a rabbit model.
Jayash SN, Hashim NM, Misran M, Baharuddin NA
PeerJ 2017;5:e3513
PeerJ 2017;5:e3513
Degradation of elastic fiber and elevated elastase expression in long head of biceps tendinopathy.
Wu YT, Su WR, Wu PT, Shen PC, Jou IM
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2017 Sep;35(9):1919-1926
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2017 Sep;35(9):1919-1926
Novel device for application of continuous mechanical tensile strain to mammalian cells.
Wada S, Kanzaki H, Narimiya T, Nakamura Y
Biology open 2017 Apr 15;6(4):518-524
Biology open 2017 Apr 15;6(4):518-524
In vitro evaluation of osteoprotegerin in chitosan for potential bone defect applications.
Jayash SN, Hashim NM, Misran M, Baharuddin NA
PeerJ 2016;4:e2229
PeerJ 2016;4:e2229
Osteopontin, E-cadherin, and β-catenin expression as prognostic biomarkers in patients with radically resected gastric cancer.
Di Bartolomeo M, Pietrantonio F, Pellegrinelli A, Martinetti A, Mariani L, Daidone MG, Bajetta E, Pelosi G, de Braud F, Floriani I, Miceli R
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 2016 Apr;19(2):412-420
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 2016 Apr;19(2):412-420
A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration.
Cheng MQ, Wahafu T, Jiang GF, Liu W, Qiao YQ, Peng XC, Cheng T, Zhang XL, He G, Liu XY
Scientific reports 2016 Apr 13;6:24134
Scientific reports 2016 Apr 13;6:24134
Intratracheal instillation of single-wall carbon nanotubes in the rat lung induces time-dependent changes in gene expression.
Fujita K, Fukuda M, Fukui H, Horie M, Endoh S, Uchida K, Shichiri M, Morimoto Y, Ogami A, Iwahashi H
Nanotoxicology 2015 May;9(3):290-301
Nanotoxicology 2015 May;9(3):290-301
Osteopontin expression in biopsies of calciphylaxis.
Magro CM, Momtahen S, Hagen JW
European journal of dermatology : EJD 2015 Jan-Feb;25(1):20-5
European journal of dermatology : EJD 2015 Jan-Feb;25(1):20-5
Nanoparticle biphasic calcium phosphate loading on gelatin-pectin scaffold for improved bone regeneration.
Nguyen TB, Min YK, Lee BT
Tissue engineering. Part A 2015 Apr;21(7-8):1376-87
Tissue engineering. Part A 2015 Apr;21(7-8):1376-87
Spinal but not cortical microglia acquire an atypical phenotype with high VEGF, galectin-3 and osteopontin, and blunted inflammatory responses in ALS rats.
Nikodemova M, Small AL, Smith SM, Mitchell GS, Watters JJ
Neurobiology of disease 2014 Sep;69:43-53
Neurobiology of disease 2014 Sep;69:43-53
Expression of osteogenic molecules in the caudate nucleus and gray matter and their potential relevance for Basal Ganglia calcification in hypoparathyroidism.
Goswami R, Millo T, Mishra S, Das M, Kapoor M, Tomar N, Saha S, Roy TS, Sreenivas V
The Journal of clinical endocrinology and metabolism 2014 May;99(5):1741-8
The Journal of clinical endocrinology and metabolism 2014 May;99(5):1741-8
Magnesium-based bone implants: immunohistochemical analysis of peri-implant osteogenesis by evaluation of osteopontin and osteocalcin expression.
Bondarenko A, Angrisani N, Meyer-Lindenberg A, Seitz JM, Waizy H, Reifenrath J
Journal of biomedical materials research. Part A 2014 May;102(5):1449-57
Journal of biomedical materials research. Part A 2014 May;102(5):1449-57
A combination of biphasic calcium phosphate scaffold with hyaluronic acid-gelatin hydrogel as a new tool for bone regeneration.
Nguyen TB, Lee BT
Tissue engineering. Part A 2014 Jul;20(13-14):1993-2004
Tissue engineering. Part A 2014 Jul;20(13-14):1993-2004
Expression of chondro-osteogenic BMPs in clinical samples of patellar tendinopathy.
Rui YF, Lui PP, Rolf CG, Wong YM, Lee YW, Chan KM
Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA 2012 Jul;20(7):1409-17
Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA 2012 Jul;20(7):1409-17
Cellular retinol-binding protein 1 (CRBP-1) regulates osteogenenesis and adipogenesis of mesenchymal stem cells through inhibiting RXRα-induced β-catenin degradation.
Xu L, Song C, Ni M, Meng F, Xie H, Li G
The international journal of biochemistry & cell biology 2012 Apr;44(4):612-9
The international journal of biochemistry & cell biology 2012 Apr;44(4):612-9
Mapping of functional epitopes of osteopontin by monoclonal antibodies raised against defined internal sequences.
Kon S, Yokosaki Y, Maeda M, Segawa T, Horikoshi Y, Tsukagoshi H, Rashid MM, Morimoto J, Inobe M, Shijubo N, Chambers AF, Uede T
Journal of cellular biochemistry 2002;84(2):420-32
Journal of cellular biochemistry 2002;84(2):420-32
Mapping of functional epitopes of osteopontin by monoclonal antibodies raised against defined internal sequences.
Kon S, Yokosaki Y, Maeda M, Segawa T, Horikoshi Y, Tsukagoshi H, Rashid MM, Morimoto J, Inobe M, Shijubo N, Chambers AF, Uede T
Journal of cellular biochemistry 2002;84(2):420-32
Journal of cellular biochemistry 2002;84(2):420-32
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Simple Western lane view shows a specific band for Osteopontin in 0.5 mg/ml of Human Breast Cancer lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Analysis of Osteopontin expression in U2OS whole cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Analysis of Osteopontin on human lung adenocarcinoma.
