LS-C745502

antibody from LSBio

Targeting: COL2A1 AOM, SEDC, STL1

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C745502

Provider

LSBio

Product name

COL2A1 / Collagen II Alpha 1 Antibody (FITC) LS-C745502

Antibody type

Polyclonal

Reactivity

Human, Bovine

Host

Rabbit

Conjugate

Green dye

Isotype

IgG

Storage

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of rabbit Anti-Collagen II Biotin Conjugated Antibody. Tissue: human bronchiolar cartilage (shown). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining. Fixation: formalin fixed paraffin embedded. Antigen retrieval: 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Primary antibody: collagen II antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen II is extracellular. Staining: Collagen II as precipitated red signal with hematoxylin purple nuclear counterstain.