MA1-90573

antibody from Invitrogen Antibodies

Targeting: IVL

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA1-90573

Provider

Invitrogen Antibodies

Product name

Involucrin Monoclonal Antibody (SY8)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-90573 detects Involucrin from non-human primate, porcine, and human samples.

Reactivity

Human, Canine, Chicken/Avian, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

SY8

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Involucrin Monoclonal Antibody (SY8)(Product # MA1-90573) and a 68kDa band corresponding to Involucrin was observed across skin derived cell lines (HaCaT and A-431) and not in others. Whole Cell Extract-WCL (30 µg lysate) of HaCaT (Lane 1), A-431 (Lane 2), HeLa (Lane 3), MDA-MB-231 (Lane 4), Raji (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.75ug/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).IVL is reported to show this pattern of expression at molecular weights >68kDa and all of these forms are not detected in the low and/or negative cell lines tested [doi:10.1242/jcs.056093; 10.1093/toxsci/kfm153]