Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30582 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Involucrin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: Raji.
- Concentration
- 1.72 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Involucrin using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Involucrin polyclonal antibody (Product # PA5-30582) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Involucrin in Various whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with Involucrin, N-term Polyclonal antibody (Product # PA5-30582) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Involucrin in Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with Involucrin, N-term Polyclonal antibody (Product # PA5-30582) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Involucrin was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Involucrin Polyclonal Antibody (Product # PA5-30582) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Involucrin Polyclonal Antibody (Product # PA5-30582). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Involucrin Polyclonal Antibody (Product # PA5-30582) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Involucrin in methanol-fixed A431 cells using an Involucrin polyclonal antibody (Product # PA5-30582) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Involucrin (Product # PA5-30582) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.