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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP7753a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP7753a, RRID:AB_2193619
- Product name
- SMAD4 Antibody (T277)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.39 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Resistance to aerobic exercise training causes metabolic dysfunction and reveals novel exercise-regulated signaling networks.
Lessard SJ, Rivas DA, Alves-Wagner AB, Hirshman MF, Gallagher IJ, Constantin-Teodosiu D, Atkins R, Greenhaff PL, Qi NR, Gustafsson T, Fielding RA, Timmons JA, Britton SL, Koch LG, Goodyear LJ
Diabetes 2013 Aug;62(8):2717-27
Diabetes 2013 Aug;62(8):2717-27
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of SMAD4 Antibody (T277)(arrow) using rabbit polyclonal SMAD4 Antibody (T277) (Cat.#AP7753a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2) (Origene Technologies).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of SMAD4 Antibody (T277)(Cat#AP7753a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
