Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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Validation data
Reference
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- Product number
- AF-223-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CD25/IL-2R alpha Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) IL-2 sR gamma is observed, less than 5% cross-reactivity with rhIL-6 sR is observed, and less than 1% cross-reactivity with rhIL-1 sRII and rhIL-2 sR beta is observed. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-2 R alpha is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.
Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.
Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab.
Interleukin-2alpha receptor in membrane lipid rafts.
Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retroviruses.
Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7221-9
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7221-9
Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.
Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7221-9
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7221-9
Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab.
Binder M, Vögtle FN, Michelfelder S, Müller F, Illerhaus G, Sundararajan S, Mertelsmann R, Trepel M
Cancer research 2007 Apr 15;67(8):3518-23
Cancer research 2007 Apr 15;67(8):3518-23
Interleukin-2alpha receptor in membrane lipid rafts.
Li QR, Ma J, Wang H, Li JS
Transplantation proceedings 2005 Jun;37(5):2395-7
Transplantation proceedings 2005 Jun;37(5):2395-7
Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retroviruses.
Sandrin V, Muriaux D, Darlix JL, Cosset FL
Journal of virology 2004 Jul;78(13):7153-64
Journal of virology 2004 Jul;78(13):7153-64
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human CD25/IL-2R alpha by Western Blot. Detection of Human CD25/IL-2R alpha by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-2 and Neutralization by Human CD25/IL-2 R alpha Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL-2 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA). The ND50 is typically 0.2-1 µg/mL.