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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MA5-23714 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD25 Monoclonal Antibody (24204)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In sandwich ELISAs, this antibody does not cross-react or interfere with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-2 R beta, rhIL-3 R alpha, rhIL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-6 R, or rhgp130.
- Antibody clone number
- 24204
- Concentration
- 0.5 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemical analysis of CD25/IL-2 Ra was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using mouse Anti-human CD25/IL-2 Ra Monoclonal Antibody (Product # MA5-23714) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-mouse IgG Secondary Antibody (yello and counterstained with DAPI (blue). Upper panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.
