- MHCD2704 - Invitrogen Antibodies CD27 antibody

Submitted references Therapeutic activity of an inhaled potent SARS-CoV-2 neutralizing human monoclonal antibody in hamsters.

Piepenbrink MS, Park JG, Oladunni FS, Deshpande A, Basu M, Sarkar S, Loos A, Woo J, Lovalenti P, Sloan D, Ye C, Chiem K, Bates CW, Burch RE, Erdmann NB, Goepfert PA, Truong VL, Walter MR, Martinez-Sobrido L, Kobie JJ
Cell reports. Medicine 2021 Mar 16;2(3):100218

Checkpoints for Autoreactive B Cells in the Peripheral Blood of Lupus Patients Assessed by Flow Cytometry.

Malkiel S, Jeganathan V, Wolfson S, Manjarrez Orduño N, Marasco E, Aranow C, Mackay M, Gregersen PK, Diamond B
Arthritis & rheumatology (Hoboken, N.J.) 2016 Sep;68(9):2210-20

Identification of a human monoclonal antibody to replace equine diphtheria antitoxin for treatment of diphtheria intoxication.

Sevigny LM, Booth BJ, Rowley KJ, Leav BA, Cheslock PS, Garrity KA, Sloan SE, Thomas W Jr, Babcock GJ, Wang Y
Infection and immunity 2013 Nov;81(11):3992-4000

CSK regulatory polymorphism is associated with systemic lupus erythematosus and influences B-cell signaling and activation.

Manjarrez-Orduño N, Marasco E, Chung SA, Katz MS, Kiridly JF, Simpfendorfer KR, Freudenberg J, Ballard DH, Nashi E, Hopkins TJ, Cunninghame Graham DS, Lee AT, Coenen MJ, Franke B, Swinkels DW, Graham RR, Kimberly RP, Gaffney PM, Vyse TJ, Behrens TW, Criswell LA, Diamond B, Gregersen PK
Nature genetics 2012 Nov;44(11):1227-30

Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen.

Smith K, Garman L, Wrammert J, Zheng NY, Capra JD, Ahmed R, Wilson PC
Nature protocols 2009;4(3):372-84

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 CSK expression varies in lymphocyte subsets and is associated with CSK genotype. (a) Gating strategy employed to distinguish monocytes and T cells (upper panel) and B cell subpopulations (bottom panel). (b) CSK expression decreases in peripheral B cells as they mature from transitional to memory cells (p = 0.030, Kruskal-Wallis test). CSK expression in peripheral T cells is low (p = 0.0047, Kruskal-Wallis test between the five cell subpopulations). (c) In naive B cells, the rs34933034 risk allele (A) is associated with increased CSK expression (29 subjects analyzed, Kruskal-Wallis test). Cell subpopulations were isolated from peripheral mononuclear cells by cell sorting as shown in (a). Expression analysis was performed by qPCR with cDNA synthesized from RNA of isolated subpopulations from mononuclear cells of non-genotyped blood donors (b) or haplotype-matched GaP subjects (c), the horizontal bar marks the median of the values.
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 The CSK risk allele is associated with increased phospho-Lyn 508 and enhanced activation of mature B cells. (a) Phosphorylated tyrosine 508 was measured by flow cytometry in naive B cells of haplotype-matched GaP donors homozygous for either allele of the rs34933034 variant; the profiles of a risk (bold) and non risk (shadowed) subject are shown, the thin line shows the isotype control; (b) pLyn 508 in carriers of the CSK risk allele. pLyn 508 was normalized to each day average (n = 27, p = 0.0136, Mann-Whitney test, see methods for correction after multiple comparison, and supplementary Figure 1 , Kruskal-Wallis test P = 0.0569). (c) A representative experiment of calcium mobilization. Basal calcium was read for 40 seconds, before activation with anti-IgM (Fab') 2 . After at least six minutes of recording, ionomycin was used as a positive control for calcium mobilization and showed no difference between genotypes (data not shown). (d) Data from eleven different ""B"" haplotype donors was normalized to one non-risk (GG) subject for comparison on each of four separate days of experiments. The area under the curve for the first 90 seconds (similar results at four minutes) is shown, (see methods for statistical analysis). (e) IgM plasma levels in 42 subjects homozygous for the B haplotype, Kruskal-Wallis test. In every graph, the horizontal line marks the median.
Conjugate: Yellow dye

MHCD2704

Antibody data