MA5-13736

antibody from Invitrogen Antibodies

Targeting: TNFRSF9 4-1BB, CD137, ILA

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-13736

Provider

Invitrogen Antibodies

Product name

CD137 Monoclonal Antibody (BBK-2), Biotin

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-13736 targets CD137 in FACS and IF applications and shows reactivity with Human samples.

Conjugate

Biotin

Antibody clone number

BBK-2

Concentration

0.2 mg/mL

References

Submitted references

Expression of CD137 on Hodgkin and Reed-Sternberg cells inhibits T-cell activation by eliminating CD137 ligand expression.

Ho WT, Pang WL, Chong SM, Castella A, Al-Salam S, Tan TE, Moh MC, Koh LK, Gan SU, Cheng CK, Schwarz H
Cancer research 2013 Jan 15;73(2):652-61

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CD137 is expressed in follicular dendritic cell tumors and in classical Hodgkin and T-cell lymphomas: diagnostic and therapeutic implications.

Anderson MW, Zhao S, Freud AG, Czerwinski DK, Kohrt H, Alizadeh AA, Houot R, Azambuja D, Biasoli I, Morais JC, Spector N, Molina-Kirsch HF, Warnke RA, Levy R, Natkunam Y
The American journal of pathology 2012 Sep;181(3):795-803

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Prediction of survival in diffuse large B-cell lymphoma based on the expression of 2 genes reflecting tumor and microenvironment.

Alizadeh AA, Gentles AJ, Alencar AJ, Liu CL, Kohrt HE, Houot R, Goldstein MJ, Zhao S, Natkunam Y, Advani RH, Gascoyne RD, Briones J, Tibshirani RJ, Myklebust JH, Plevritis SK, Lossos IS, Levy R
Blood 2011 Aug 4;118(5):1350-8

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CD137 enhances monocyte-ICAM-1 interactions in an E-selectin-dependent manner under flow conditions.

Quek BZ, Lim YC, Lin JH, Tan TE, Chan J, Biswas A, Schwarz H
Molecular immunology 2010 May;47(9):1839-47

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CD137 ligand mediates opposite effects in human and mouse NK cells and impairs NK-cell reactivity against human acute myeloid leukemia cells.

Baessler T, Charton JE, Schmiedel BJ, Grünebach F, Krusch M, Wacker A, Rammensee HG, Salih HR
Blood 2010 Apr 15;115(15):3058-69

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Induction of proliferation and monocytic differentiation of human CD34+ cells by CD137 ligand signaling.

Jiang D, Yue PS, Drenkard D, Schwarz H
Stem cells (Dayton, Ohio) 2008 Sep;26(9):2372-81

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Molecular mechanisms and gene regulation of melphalan- and hyperthermia-induced apoptosis in Ewing sarcoma cells.

Krause C, Klüttermann K, Mauz-Körholz C
Anticancer research 2008 Sep-Oct;28(5A):2585-93

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Constitutive expression of functional 4-1BB (CD137) ligand on carcinoma cells.

Salih HR, Kosowski SG, Haluska VF, Starling GC, Loo DT, Lee F, Aruffo AA, Trail PA, Kiener PA
Journal of immunology (Baltimore, Md. : 1950) 2000 Sep 1;165(5):2903-10

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Constitutive expression of functional 4-1BB (CD137) ligand on carcinoma cells.

Salih HR, Kosowski SG, Haluska VF, Starling GC, Loo DT, Lee F, Aruffo AA, Trail PA, Kiener PA
Journal of immunology (Baltimore, Md. : 1950) 2000 Sep 1;165(5):2903-10

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD137 in PBMC cells stimulated with PHA for 3 days (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-13739) at a dilution of 1 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4ºC in the dark. FACS analysis was performed using 400 µL of cell buffer.

Conjugate

Biotin