MAB838-100

antibody from R&D Systems

Targeting: TNFRSF9 4-1BB, CD137, ILA

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB838-100

Provider

R&D Systems

Product name

Human 4-1BB/TNFRSF9/CD137 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human 4-1BB/TNFRSF9/CD137 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) 4-1BB, recombinant human (rh) CD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFas, rhGITR, rmGITR, rhHVEM, rhLTR beta , rmLTR beta , rhNGFR, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q07011


Isotype

IgG

Antibody clone number

145501

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of 4-1BB/TNFRSF9/ CD137 in Human CD3⁺ PBMCs by Flow Cytometry. Human CD3⁺ peripheral blood mononuclear cells were treated for 48 hours with 5 μg/mL PHA then stained with Mouse Anti-Human 4-1BB/TNFRSF9/CD137 Mono-clonal Antibody (Catalog # MAB838, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂ Secondary Antibody (Catalog # F0101B).