Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 62-1379-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD137 (4-1BB) Monoclonal Antibody (4B4 (4B4-1)), Super Bright™ 436, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 4B4 (4B4-1) monoclonal antibody reacts with human CD137 (also known as 4-1BB or TNFRSF9), which is an inducible member of the TNFR family of costimulatory molecules expressed on T cells, natural killer cells, dendritic cells, granulocytes, and mast cells. Involved in recruiting TNFR-associated factors (TRAF) 1 and 2, CD137 signaling plays a role in T cell activation, maintaining the survival of activated and CD8 memory T cells, as well as suppressing myelopoiesis and dendritic cell development. Stimulation of this receptor has also been shown to promote expansion of CD4+CD25+ T regulatory cells ex vivo. The ligand for CD137, 4-1BBL, is found on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells.
- Antibody clone number
- 4B4 (4B4-1)
- Concentration
- 5 µL/Test
Submitted references Neoantigens retention in patient derived xenograft models mediates autologous T cells activation in ovarian cancer.
Want MY, Konstorum A, Huang RY, Jain V, Matsueda S, Tsuji T, Lugade A, Odunsi K, Koya R, Battaglia S
Oncoimmunology 2019;8(6):e1586042
Oncoimmunology 2019;8(6):e1586042
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of 3-day Con A-stimulated normal human peripheral blood cells with Anti-Human/Non-Human Primate CD25 APC (Product # 17-0257-42) and Mouse IgG1 K Isotype Control Super Bright 436 (Product # 62-4714-82) (left) or Anti-Human CD137 (4-1BB) Super Bright 436 (right). Total viable cells were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 10.1080/2162402X.2019.1586042-F0004 Figure 4. Neoantigen recognition by autologous T cells in-vitro . a) Ratio of IFN-gamma production in patient's T cells at different time points upon coculture with mutated (MUT) or WT peptide-stimulated autologous APCs. Y axis indicates the ratio IFN-gamma Mut(pg/ml)/IFN-gamma WT(pg/ml) measured via ELISA. Peptides with a ratio > 1 are highlighted in red boxes and utilized for subsequent experiments. ND = no IFN-gamma production detected. b) Flow cytometry analysis of CD137 + T cells expressed as percent of activated CD8 + T cells after overnight stimulation with 10 um mutated (MUT) or WT peptides. c) Flow plots of CD137 + /CD8 + T cells in response to coculture with APC pulsed with mutated (MUT) or WT peptides. d) Line plot showing the changes in variant allele frequency of the six neoantigenic mutations common across the primary, P0 and P1 tumors. Orange lines represent mutations present in all samples, black dotted mutations indicate those present in P0 and P1 but absent (VAF = 0) in the primary tumor.