R32714

antibody from NSJ Bioreagents

Targeting: BSG CD147, EMMPRIN, EMPRIN, OK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R32714

Provider

NSJ Bioreagents

Product name

CD147 / Emmprin / Basigin Antibody

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Concentration

0.5mg/ml

Storage

After reconstitution, the Basigin / CD147 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) placenta, 3) Jurkat, 4) U-2 OS, 5) K562, 6) MCF-7, 7) COLO-320, 8) HepG2 and 9) PANC-1 lysate with CD147 antibody. Expected molecular weight: 27-66 kDa depending on level of glycosylation.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC staining of FFPE human A549 cells with CD147 antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC staining of FFPE human HeLa cells with CD147 antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human tonsil tissue with CD147 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human intestinal cancer tissue with CD147 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human lung cancer tissue with CD147 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human intestinal cancer with CD147 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HeLa cells with CD147 antibody at 1ug/10^6 cells (cells blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD147 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human A549 cells with CD147 antibody at 1ug/10^6 cells (cells blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD147 antibody.