Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-17716 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD163 Monoclonal Antibody (GHI/61)
- Antibody type
- Monoclonal
- Description
- MA5-17716 targets CD163/M130 in FACS, IHC (F), IP, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- GHI/61
- Concentration
- 1 mg/mL
Submitted references Age-Related Changes in Immune Cells of the Human Cochlea.
Noble KV, Liu T, Matthews LJ, Schulte BA, Lang H
Frontiers in neurology 2019;10:895
Frontiers in neurology 2019;10:895
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded human spleen sections at 400x magnification (A) and human tonsil sections at 200x (B) 400x (C) magnification with DAPI counterstain. Primary antibody used was a CD163/M130 monoclonal antibody (clone GHI/61) at a 1:500 dilution, followed by secondary antibody was a 488 fluorescent conjugated Goat anti-mouse Ig at a 1:500 dilution and Normal Goat Serum (blocking)