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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 353314 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#353314, RRID:AB_2571971
- Product name
- PerCP/Cy5.5 anti-human Perforin
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Antibody clone number
- B-D48
- Vial size
- 100 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Human peripheral blood lymphocytes were surface stained with CD8 FITC, fixed with Fixation Buffer, permeabilized with Intracellular Staining Permeabilization Wash Buffer (10X), and then intracellularly stained with Perforin (clone B-D48) PerCp/Cy5.5 (top), or mouse IgG1, κ PerCp/Cy5.5 isotype control (bottom).
