308110

antibody from BioLegend

Targeting: PRF1 HPLH2, P1, PFP

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

308110

Provider

BioLegend

Proper citation

BioLegend Cat#308110, RRID:AB_493254

Product name

Alexa Fluor® 647 anti-human Perforin [dG9]

Antibody type

Monoclonal

Antigen

Purified granules from the human lymphoma cell line

Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Reactivity

Human, Bovine, Feline

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Vial size

100 tests

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Submitted references

Docking of lytic granules at the immunological synapse in human CTL requires Vti1b-dependent pairing with CD3 endosomes.

Qu B, Pattu V, Junker C, Schwarz EC, Bhat SS, Kummerow C, Marshall M, Matti U, Neumann F, Pfreundschuh M, Becherer U, Rieger H, Rettig J, Hoth M
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):6894-904

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Different NK cell-activating receptors preferentially recruit Rab27a or Munc13-4 to perforin-containing granules for cytotoxicity.

Wood SM, Meeths M, Chiang SC, Bechensteen AG, Boelens JJ, Heilmann C, Horiuchi H, Rosthøj S, Rutynowska O, Winiarski J, Stow JL, Nordenskjöld M, Henter JI, Ljunggren HG, Bryceson YT
Blood 2009 Nov 5;114(19):4117-27

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood mononuclear cells intracellular stained with DG9 Alexa Fluor® 647

Conjugate

Red dye