PA5-29625

antibody from Invitrogen Antibodies

Targeting: TNFSF10 Apo-2L, CD253, TL2, TRAIL

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29625

Provider

Invitrogen Antibodies

Product name

TRAIL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: human TNFSF10-transfected 293T cells.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TRAIL Polyclonal Antibody detects TNFSF10 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human TNFSF10-transfected 293T cells.12 % SDS-PAGE. TRAIL Polyclonal Antibody (Product # PA5-29625) dilution: 1:5,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-TRAIL Polyclonal Antibody (Product # PA5-29625) and a 28 kDa band corresponding to Tumor necrosis factor ligand superfamily member 10 was observed along with an uncharacterized band (*) around 60 kDa. Whole cell extracts (40 µg lysate) of A-431 (Lane 1), A-431 treated with Protein Transport Inhibitor (PTI) cocktail (1X, 4 hours) (Lane 2), HT-29 (Lane 3), HT-29 treated with Protein Transport Inhibitor (PTI) cocktail (1X, 4 hours) (Lane 4), U-2 OS (Lane 5), U-2 OS treated with Protein Transport Inhibitor (PTI) cocktail (1X, 4 hours) (Lane 6) and BeWo (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20,000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). It is reported that HT-29 and U-2 OS express very low levels of TRAIL. Accordingly, relative expression was observed in A431 treated with PTI cocktail in comparison with HT-29 treated with PTI cocktail and U-2 OS treated with PTI cocktail.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TRAIL Polyclonal Antibody detects TNFSF10 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human TNFSF10-transfected 293T cells.12 % SDS-PAGE. TRAIL Polyclonal Antibody (Product # PA5-29625) dilution: 1:5,000.