Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-13567 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD37 Monoclonal Antibody (IPO-24)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-13567 targets CD37 in FACS, IF, and IHC (F) applications and shows reactivity with Human samples.
- Antibody clone number
- IPO-24
- Concentration
- 0.2 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Flow cytometry analysis of CD37 in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-13567) at a dilution of 2 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4ºC in the dark. FACS analysis was performed using 400 µL of cell buffer.