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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB6322-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD40/TNFRSF5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CD40 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhNGF R, rhOPG, rhRANK, rhTAJ, rhTNF RI, or rhTNF RII is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P25942- Isotype
- IgG
- Antibody clone number
- 82102
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Structural Diversity and Anticancer Activity of Marine-Derived Elastase Inhibitors: Key Features and Mechanisms Mediating the Antimetastatic Effects in Invasive Breast Cancer.
Up-Regulated Expression of Matrix Metalloproteinases in Endothelial Cells Mediates Platelet Microvesicle-Induced Angiogenesis.
Circulating CXCR5+CXCR3+PD-1lo Tfh-like cells in HIV-1 controllers with neutralizing antibody breadth.
A soluble form of IL-27Rα is a natural IL-27 antagonist.
ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
Circulating myeloid dendritic cells of advanced cancer patients result in reduced activation and a biased cytokine profile in invariant NKT cells.
IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
Al-Awadhi FH, Paul VJ, Luesch H
Chembiochem : a European journal of chemical biology 2018 Apr 16;19(8):815-825
Chembiochem : a European journal of chemical biology 2018 Apr 16;19(8):815-825
Up-Regulated Expression of Matrix Metalloproteinases in Endothelial Cells Mediates Platelet Microvesicle-Induced Angiogenesis.
Sun C, Feng SB, Cao ZW, Bei JJ, Chen Q, Zhao WB, Xu XJ, Zhou Z, Yu ZP, Hu HY
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;41(6):2319-2332
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;41(6):2319-2332
Circulating CXCR5+CXCR3+PD-1lo Tfh-like cells in HIV-1 controllers with neutralizing antibody breadth.
Martin-Gayo E, Cronin J, Hickman T, Ouyang Z, Lindqvist M, Kolb KE, Schulze Zur Wiesch J, Cubas R, Porichis F, Shalek AK, van Lunzen J, Haddad EK, Walker BD, Kaufmann DE, Lichterfeld M, Yu XG
JCI insight 2017 Jan 26;2(2):e89574
JCI insight 2017 Jan 26;2(2):e89574
A soluble form of IL-27Rα is a natural IL-27 antagonist.
Dietrich C, Candon S, Ruemmele FM, Devergne O
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5382-9
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5382-9
ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
Weissmüller S, Semmler LY, Kalinke U, Christians S, Müller-Berghaus J, Waibler Z
Blood 2012 Jun 28;119(26):6268-77
Blood 2012 Jun 28;119(26):6268-77
Circulating myeloid dendritic cells of advanced cancer patients result in reduced activation and a biased cytokine profile in invariant NKT cells.
van der Vliet HJ, Wang R, Yue SC, Koon HB, Balk SP, Exley MA
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7287-93
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7287-93
IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 1;178(5):2872-82
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 1;178(5):2872-82
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD40/TNFRSF5 Mouse by Western Blot. Western blot shows lysates of human spleen tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD40/TNFRSF5 Monoclonal Antibody (Catalog # MAB6322) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CD40/TNFRSF5 at approximately 40 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by CD40 Ligand/TNFSF5 and Neutralization by Human CD40/TNFRSF5 Antibody. In the presence of Recombinant Human IL-4 (Catalog # 204-IL), Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6245-CL) stimulates proliferation in human B cell enriched peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CD40 Ligand/TNFSF5 (10 µg/mL) is neutralized (green line) by increasing concentrations of Human CD40/TNFRSF5 Monoclonal Antibody (Catalog # MAB6322). At 5 µg/mL, this anti-hCD40 antibody will neutralize approximately 80% of Recombinant Human CD40 Ligand/TNFSF5 -induced proliferation in the presence of Recombinant Human IL-4 (20 ng/mL).
