Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 11-0839-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD83 Monoclonal Antibody (HB15e), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HB15e monoclonal antibody reacts with human CD83, a 45 kDa transmembrane glycoprotein. CD83, a member of the Ig superfamily, is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells as well as some proliferating lymphocytes, and human cell lines express this antigen. While the function of CD83 is unclear, it can serve as a useful marker for mature human blood dendritic cells. Applications Reported: The HB15e antibody has been reported for use in flow cytometric analysis. Applications Tested: This HB15e antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- HB15e
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Hepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism.
Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.
Novel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses.
Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen.
Early secreted antigenic target of 6-kDa protein of Mycobacterium tuberculosis primes dendritic cells to stimulate Th17 and inhibit Th1 immune responses.
Dendritic cells derived from hemozoin-loaded monocytes display a partial maturation phenotype that promotes HIV-1 trans-infection of CD4+ T cells and virus replication.
van Montfoort N, van der Aa E, van den Bosch A, Brouwers H, Vanwolleghem T, Janssen HLA, Javanbakht H, Buschow SI, Woltman AM
Journal of virology 2016 Jul 15;90(14):6187-6199
Journal of virology 2016 Jul 15;90(14):6187-6199
Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.
Miles B, Zakhary I, El-Awady A, Scisci E, Carrion J, O'Neill JC, Rawlings A, Stern JK, Susin C, Cutler CW
Infection and immunity 2014 Jan;82(1):101-11
Infection and immunity 2014 Jan;82(1):101-11
Novel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses.
Preston SG, Majtán J, Kouremenou C, Rysnik O, Burger LF, Cabezas Cruz A, Chiong Guzman M, Nunn MA, Paesen GC, Nuttall PA, Austyn JM
PLoS pathogens 2013;9(6):e1003450
PLoS pathogens 2013;9(6):e1003450
Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen.
Miles B, Scisci E, Carrion J, Sabino GJ, Genco CA, Cutler CW
Journal of leukocyte biology 2013 Aug;94(2):281-9
Journal of leukocyte biology 2013 Aug;94(2):281-9
Early secreted antigenic target of 6-kDa protein of Mycobacterium tuberculosis primes dendritic cells to stimulate Th17 and inhibit Th1 immune responses.
Wang X, Barnes PF, Huang F, Alvarez IB, Neuenschwander PF, Sherman DR, Samten B
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 15;189(6):3092-103
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 15;189(6):3092-103
Dendritic cells derived from hemozoin-loaded monocytes display a partial maturation phenotype that promotes HIV-1 trans-infection of CD4+ T cells and virus replication.
Diou J, Tardif MR, Barat C, Tremblay MJ
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):2899-907
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):2899-907
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of 1 day PHA-stimulated human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD83 FITC (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye