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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- NB100-1498 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1498, RRID:AB_2187047
- Product name
- Goat Polyclonal Serpin E1/PAI-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both reported isoforms.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Activation of mast cells by incorporation of cholesterol into rafts.
Activation of mast cells by incorporation of cholesterol into rafts.
Baumruker T, Csonga R, Pursch E, Pfeffer A, Urtz N, Sutton S, Bofill-Cardona E, Cooke M, Prieschl E
International immunology 2003 Oct;15(10):1207-18
International immunology 2003 Oct;15(10):1207-18
Activation of mast cells by incorporation of cholesterol into rafts.
Baumruker T, Csonga R, Pursch E, Pfeffer A, Urtz N, Sutton S, Bofill-Cardona E, Cooke M, Prieschl E
International immunology 2003 Oct;15(10):1207-18
International immunology 2003 Oct;15(10):1207-18
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Serpin E1/PAI-1 Antibody [NB100-1498] - (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Serpin E1/PAI-1 Antibody [NB100-1498] - Staining of A549 (A), HepG2 (B) and HeLa (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Serpin E1/PAI-1 Antibody [NB100-1498] - (1.5ug/ml) as the reporter used with capture rabbit antibody (2.5ug/ml).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NB100-1498] - (4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
