Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [10]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX102424 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102424, RRID:AB_2038197
- Product name
- Tyrosine Hydroxylase antibody [N1C1]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Zebrafish
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE GTX102424 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody (GTX102424) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Tyrosine hydroxylase antibody [N1C1] detects tyrosine hydroxylase protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Tyrosine hydroxylase antibody [N1C1] [GTX102424] diluted at 1:1000. Blue: DAPI
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:250.Red: SOX2, stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm in rat adrenal gland by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Tyrosine Hydroxylase antibody [N1C1] (GTX102424) dilution: 1:100.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Tyrosine Hydroxylase antibody [N1C1] (GTX102424) dilution: 1:100.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.