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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- AF7566 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Tyrosine Hydroxylase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse and rat Tyrosine Hydroxylase in Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human TPH1 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P07101- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Tyrosine Hydroxylase by Western Blot. Western blot shows lysates of human adrenal gland tissue, mouse adrenal gland tissue, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Tyrosine Hydroxylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7566) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Tyrosine Hydroxylase at approximately 56-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Rat Tyrosine Hydroxylase by Simple WesternTM. Simple Western lane view shows lysates of PC-12 rat adrenal pheochromocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Tyrosine Hydroxylase at approximately 59 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat Tyrosine Hydroxylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7566) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Tyrosine Hydroxylase in D3 Mouse Embryonic Stem Cells. Tyrosine Hydroxylase was detected in immersion fixed D3 mouse embryonic stem cells using Sheep Anti-Human Tyrosine Hydroxylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7566) at 10 µg/mL for 3 hours at room temperature. Cells were differentiated into dopaminergic neurons using R&D Systems Human/Mouse Dopaminergic Neuron Differentiation Kit (Catalog # SC001B). Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to dopaminergic neurons. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
