Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- UM800105 - Provider product page
- Provider
- OriGene
- Product name
- NAPSA mouse monoclonal antibody,clone UMAB213
- Antibody type
- Monoclonal
- Description
- NAPSA mouse monoclonal antibody,clone UMAB213
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NAPSA
- Isotype
- IgG
- Antibody clone number
- UMAB213
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human lung tissue located within the normal limits of tumor using anti-NAPSA mouse monoclonal antibody. HIER pretreatment was with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800105 was diluted 1:40000 and detected with HRP-DAB. Strong cytoplasmic and membrane staining seen in the macrophages and pneumocyte. Other cell types in lung are negative.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded carcinoma of human lung tissue using anti-NAPSA mouse monoclonal antibody. HIER pretreatment with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800105 was diluted 1:40000 and detected with HRP secondary and DAB chromogen. Strong cytoplasmic and membraneous staining seen in the tumor cells.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NAPSA mouse monoclonal antibody (UM800105). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:200)
- Validation comment
- 10K-CHIP