- 33-7000 - Invitrogen Antibodies CDKN1A antibody

Submitted references Increased p53 signaling impairs neural differentiation in HUWE1-promoted intellectual disabilities.

Aprigliano R, Aksu ME, Bradamante S, Mihaljevic B, Wang W, Rian K, Montaldo NP, Grooms KM, Fordyce Martin SL, Bordin DL, Bosshard M, Peng Y, Alexov E, Skinner C, Liabakk NB, Sullivan GJ, Bjørås M, Schwartz CE, van Loon B
Cell reports. Medicine 2021 Apr 20;2(4):100240

CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1.

Li S, Liu N, Piao J, Meng F, Li Y
OncoTargets and therapy 2020;13:12383-12395

Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis.

Ye G, Xie Z, Zeng H, Wang P, Li J, Zheng G, Wang S, Cao Q, Li M, Liu W, Cen S, Li Z, Wu Y, Ye Z, Shen H
Cell death & disease 2020 Sep 17;11(9):775

Parthenolide inhibits the proliferation and induces the apoptosis of human uveal melanoma cells.

Che ST, Bie L, Li X, Qi H, Yu P, Zuo L
International journal of ophthalmology 2019;12(10):1531-1538

Generation of Human Fatty Livers Using Custom-Engineered Induced Pluripotent Stem Cells with Modifiable SIRT1 Metabolism.

Collin de l'Hortet A, Takeishi K, Guzman-Lepe J, Morita K, Achreja A, Popovic B, Wang Y, Handa K, Mittal A, Meurs N, Zhu Z, Weinberg F, Salomon M, Fox IJ, Deng CX, Nagrath D, Soto-Gutierrez A
Cell metabolism 2019 Aug 6;30(2):385-401.e9

Pooled screening for antiproliferative inhibitors of protein-protein interactions.

Nim S, Jeon J, Corbi-Verge C, Seo MH, Ivarsson Y, Moffat J, Tarasova N, Kim PM
Nature chemical biology 2016 Apr;12(4):275-81

Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration.

Irvine KM, Skoien R, Bokil NJ, Melino M, Thomas GP, Loo D, Gabrielli B, Hill MM, Sweet MJ, Clouston AD, Powell EE
World journal of gastroenterology 2014 Dec 21;20(47):17851-62

Targeting CDH17 suppresses tumor progression in gastric cancer by downregulating Wnt/β-catenin signaling.

Qiu HB, Zhang LY, Ren C, Zeng ZL, Wu WJ, Luo HY, Zhou ZW, Xu RH
PloS one 2013;8(3):e56959

A study of the combination of triamcinolone and 5-fluorouracil in modulating keloid fibroblasts in vitro.

Huang L, Cai YJ, Lung I, Leung BC, Burd A
Journal of plastic, reconstructive & aesthetic surgery : JPRAS 2013 Sep;66(9):e251-9

MicroRNA-224 negatively regulates p21 expression during late neoplastic progression in inflammatory bowel disease.

Olaru AV, Yamanaka S, Vazquez C, Mori Y, Cheng Y, Abraham JM, Bayless TM, Harpaz N, Selaru FM, Meltzer SJ
Inflammatory bowel diseases 2013 Mar;19(3):471-80

Optimization of immunolocalization of cell cycle proteins in human corneal endothelial cells.

He Z, Campolmi N, Ha Thi BM, Dumollard JM, Peoc'h M, Garraud O, Piselli S, Gain P, Thuret G
Molecular vision 2011;17:3494-511

Low-dose 5-fluorouracil induces cell cycle G2 arrest and apoptosis in keloid fibroblasts.

Huang L, Wong YP, Cai YJ, Lung I, Leung CS, Burd A
The British journal of dermatology 2010 Dec;163(6):1181-5

22-oxa-1,25-dihydroxyvitamin D3 efficiently inhibits tumor growth in inoculated mice and primary histoculture of cholangiocarcinoma.

Seubwai W, Wongkham C, Puapairoj A, Okada S, Wongkham S
Cancer 2010 Dec 1;116(23):5535-43

Clinical and immunohistochemical features associated with a response to bortezomib in patients with multiple myeloma.

Dawson MA, Opat SS, Taouk Y, Donovan M, Zammit M, Monaghan K, Horvath N, Roberts AW, Prince HM, Hertzberg M, McLean CA, Spencer A
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 15;15(2):714-22

The abnormalities in the p53/p21WAF1 pathway have a significant role in the pathogenesis and progression of gastrointestinal stromal tumors.

Chou YP, Lin JW, Wang CC, Chiu YC, Huang CC, Chuah SK, Tai MH, Yi LN, Lee CM, Changchien CS, Hu TH
Oncology reports 2008 Jan;19(1):49-56

Elevated p21 expression is associated with poor prognosis of rectal stromal tumors after resection.

Hu TH, Tai MH, Chuah SK, Chen HH, Lin JW, Huang HY, Chou YP, Yi LN, Kuo CM, Changchien CS
Journal of surgical oncology 2008 Aug 1;98(2):117-23

Activation of p53-dependent growth suppression in human cells by mutations in PTEN or PIK3CA.

Kim JS, Lee C, Bonifant CL, Ressom H, Waldman T
Molecular and cellular biology 2007 Jan;27(2):662-77

Immunohistochemical analysis of non-small cell lung cancer: correlation with clinical parameters and prognosis.

Yoo J, Jung JH, Lee MA, Seo KJ, Shim BY, Kim SH, Cho DG, Ahn MI, Kim CH, Cho KD, Kang SJ, Kim HK
Journal of Korean medical science 2007 Apr;22(2):318-25

Potent iron chelators increase the mRNA levels of the universal cyclin-dependent kinase inhibitor p21(CIP1/WAF1), but paradoxically inhibit its translation: a potential mechanism of cell cycle dysregulation.

Le NT, Richardson DR
Carcinogenesis 2003 Jun;24(6):1045-58

Genome-wide expression profiling of 8-chloroadenosine- and 8-chloro-cAMP-treated human neuroblastoma cells using radioactive human cDNA microarray.

Park GH, Choe J, Choo HJ, Park YG, Sohn J, Kim MK
Experimental & molecular medicine 2002 Jul 31;34(3):184-93

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of p21 was performed by loading 20 µg of HeLa (lane1), HeLa treated for 10 minutes with 100 ng/mL of EGF (lane2), MCF7 (lane3), COS-7 (lane4), and HCT 116 (lane5) cell lysates using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. p21 was detected at ~21 kDa using p21 Mouse Monoclonal Antibody (Product # 33-7000) at 0.5 µg - 1 µg/mL in 2.5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse IgG - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of p21 was achieved by transfecting MCF7 with p21 specific validated siRNAs (Silencer® select Product # s417). Western blot analysis (Fig. a) was performed using whole cell extracts from the p21 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with p21 Monoclonal Antibody (EA10) (Product # 33-7000, 1:500 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to p21.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of p21 was done on 70% confluent log phase U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with p21 Mouse Monoclonal Antibody (Product # 33-7000) at 2 µg - 4 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin (Product # A12381). Panel d is a merged image showing nuclear localization of p21. Panel e shows no primary antibody. The images were captured at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse anti-p21 (EA10) stained lung squamous cell carcinoma.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Optimized immunolocalization of three cyclin dependent kinase inhibitors p16, p21, and p27 in endothelial cells on flat-mounted whole human corneas. Only the best combination of fixative and epitope retrieval is shown for each antibody (see Table 4 ). Original magnification 40x except insets (96x). Bar 10 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 CDH17 activates multiple signal transduction pathways. A. Expression of beta-catenin, GSK-3beta, p- GSK-3beta, Rb, Cyclin D1were compared between shCDH17 and Mock both in AGS and MKN-45 cells by Western blot analysis. MDM2, p53 and p21 also were evaluated. beta-Actin was used as a loading control. B. TOPflash/FOPflash reporter assay shows that Wnt signaling re-activated after CDH17 restoration in AGS and MKN-45. (* P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 MSCs located in the articular soft tissue of AS patients displayed remarkable characteristics of senescence. a Tissues from AS patients displayed stronger SA-beta-gal staining (black arrow) than those from non-AS donors (scale bar = 100 um). b - d Immunofluorescence detection indicated increased levels of p53 ( b ), p21 ( c ), and p16 ( d ) in MSCs in articular soft tissue samples from AS patients than in those from non-AS patients (scale bar = 40 um). All experiments were performed three independent times ( n = 4).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 p53 accumulation and activation, caused by HUWE1 p.G4310R, perturb the cell cycle and impair proliferation in JMS patient-derived cells (A) Immunoblot analysis of the HUWE1, p53, and p21 protein levels in healthy control LCs and LCs from two JMS patients (JMS1 and JMS2). p53 protein levels relative to tubulin, serving as loading control, are indicated. (B) In vitro ubiquitination of purified recombinant p53 with increasing amounts of wild-type (WT) and p.G4310R HECT proteins. (C) In silico analysis of difference in the folding free energy change (DeltaDeltaG) of WT and G4310R HUWE1 using the indicated prediction tools. (D) Cell-cycle distribution determined by flow cytometry of healthy control, JMS1, and JMS2 LCs. (E) Fraction of annexin V-positive apoptotic cells measured by flow cytometry. (F) Proliferation rate of healthy control, JMS1, and JMS2 LCs. All error bars indicate mean +- SEM (n >= 3, biological replicates). Statistical significance was determined by two-way ANOVA with Tukey post-test (D) and Bonferroni post-test (F); one-way ANOVA with Bonferroni post-test; *p

33-7000

Antibody data