Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-37716 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Rb (Ser807) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is NIH-3T3 cells; suggested positive control for IHC is human breast carcinoma.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from NIH-3T3 cells, untreated or treated with UV, using Rb (pSer807) polyclonal antibody (Product # PA5-37716).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from NIH-3T3 cells, untreated or treated with UV, using Rb (pSer807) polyclonal antibody (Product # PA5-37716).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Phospho-Rb (Ser807) Polyclonal Antibody (Product # PA5-37716) and a 106 kDa band corresponding to Phospho-Rb along with uncharacterised band (*) between 60-80 kDa were observed across treatment panel. Nuclear enriched extracts (40 µg lysate) of LNCaP (Lane 1), LNCaP treated with Nocodazole (83 nm, 24hours) (Lane 2) exposed for 5 seconds, K-562 (Lane 3), K-562 treated with Nocodazole (83 nm, 24hours) (Lane 4) exposed for 45 seconds, were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-Rb (Ser807) in paraffin-embedded Human breast carcinoma tissue using Phospho-Rb (Ser807) Polyclonal Antibody (Product # PA5-37716) (left) or the same antibody preincubated with blocking peptide (right).