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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- ABIN1107491 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Histone Deacetylase 1 (HDAC1) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- Syntehtic peptide (C-KPEAKGVKEEVK) from the C-Terminus of Human HDAC1 (NP_004955.2). Genename: HDAC1
- Description
- Affinity Chromatography
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Goat
- Epitope
- C-Term
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP31951PU-N HDAC1 antibody staining of HeLa lysate at 0.5 μg/ml. (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ChIP of 2 μg AP31951PU-N with 1ug MCF7 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.
