TA505093

antibody from OriGene

Targeting: CBLC CBL-3, CBL-SL, RNF57

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505093

Provider

OriGene

Proper citation

OriGene Cat#TA505093, RRID:AB_2622832

Product name

CBLC mouse monoclonal antibody, clone OTI4E3 (formerly 4E3)

Antibody type

Monoclonal

Description

CBLC mouse monoclonal antibody, clone OTI4E3 (formerly 4E3)

Host

Mouse

Conjugate

Unconjugated

Epitope

CBLC

Isotype

IgG

Antibody clone number

OTI4E3

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBLC (RC205130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBLC.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CBLC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC205130 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CBLC antibody(TA505093), and then analyzed by flow cytometry.

Validation comment

FC