LS-B57

antibody from LSBio

Targeting: SFRP1 FRP, FRP-1, SARP2

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B57

Provider

LSBio

Proper citation

LifeSpan Cat#LS-B57, RRID:AB_2270223

Product name

IHC-plus™ SFRP1 Antibody LS-B57

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Affinity Purified anti-Human SFRP1 antibody was used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG (p/n 611-1302) is suitable for secondary antibody detection.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-SFRP1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Affinity Purified anti-Human SFRP1 antibody was used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG (p/n 611-1302) is suitable for secondary antibody detection.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Affinity Purified anti-Human SFRP1 antibody was used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG (p/n 611-1302) is suitable for secondary antibody detection.