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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [11]
- Immunohistochemistry [4]
- Flow cytometry [2]
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- Product number
- MA5-17145 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCNA Monoclonal Antibody (7H4F8)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17145 targets PCNA in FACS, IHC, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 7H4F8
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCNA using a PCNA monoclonal antibody (Product # MA5-17145) against a human PCNA recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCNA in A431 (lane 1), HeLa (lane 2), HepG2 (lane 3), Raji (lane 4), and MOLT4 (lane 5) cell lysate using PCNA monoclonal antibody (Product # MA5-17145).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCNA using PCNA monoclonal antibody (Product # MA5-17145) in HEK293 (1) and PCNA (AA: 53-196) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCNA using PCNA monoclonal antibody (Product # MA5-17145) in A431 (1), HEK293 (2), HeLa (3), HepG2 (4), Raji (5), MOLT4 (6), COS-7 (7), and MCF-7 (8) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using PCNA Monoclonal Antibody (7H4F8) (Product # MA5-17145) and a 33 kDa band corresponding to PCNA was observed across in all tested tissue lysates. Tissue extracts (30 µg lysate) of Mouse Testis (Lane 1), Mouse Spleen (Lane 2), Mouse Thymus (Lane 3), Rat Testis (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:10,000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCNA in A431 (lane 1), HeLa (lane 2), HepG2 (lane 3), Raji (lane 4), and MOLT4 (lane 5) cell lysate using PCNA monoclonal antibody (Product # MA5-17145).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of PCNA was achieved by transfecting HeLa with PCNA specific siRNAs (Silencer® select Product # S10133, S10134). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the PCNA knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with PCNA Monoclonal Antibody (7H4F8) (Product # MA5-17145, 1:1000 ) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PCNA.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PCNA Monoclonal Antibody (7H4F8)(Product # MA5-17145) and a 32kDa band corresponding to PCNA was observed across all tested cell lines. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), Jurkat (Lane 3), HEK-293 (Lane 4), NIH/3T3 (Lane 5), A549 (Lane 6), SH-SY5Y (Lane 7), PC-12 (Lane 8) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody ( 1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCNA using a PCNA monoclonal antibody (Product # MA5-17145) against a human PCNA recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCNA using PCNA monoclonal antibody (Product # MA5-17145) in HEK293 (1) and PCNA (AA: 53-196) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCNA using PCNA monoclonal antibody (Product # MA5-17145) in A431 (1), HEK293 (2), HeLa (3), HepG2 (4), Raji (5), MOLT4 (6), COS-7 (7), and MCF-7 (8) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PCNA monoclonal antibody (Product # MA5-17145) with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PCNA monoclonal antibody (Product # MA5-17145) with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PCNA monoclonal antibody (Product # MA5-17145) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PCNA monoclonal antibody (Product # MA5-17145) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MOLT4 cells using PCNA monoclonal antibody (Product # MA5-17145) (green) and negative control (purple).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MOLT4 cells using PCNA monoclonal antibody (Product # MA5-17145) (green) and negative control (purple).
