NB100-478

antibody from Novus Biologicals

Targeting: PCNA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

NB100-478

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-478, RRID:AB_10002563

Product name

Mouse Monoclonal PCNA Antibody

Antibody type

Monoclonal

Description

Protein G purified. This reacts with the interdomain connector loop of human PCNA, other not determined.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker.

Malkas LH, Herbert BS, Abdel-Aziz W, Dobrolecki LE, Liu Y, Agarwal B, Hoelz D, Badve S, Schnaper L, Arnold RJ, Mechref Y, Novotny MV, Loehrer P, Goulet RJ, Hickey RJ
Proceedings of the National Academy of Sciences of the United States of America 2006 Dec 19;103(51):19472-7

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PCNA Antibody (339) [NB100-478]

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PCNA Antibody (339) [NB100-478] - A: Jurkat , B: Raji, C: 293T, D: A431, E: HeLa, F: HepG2, G: H1299, H: HCT116, I: MCF-7, J: NT2D1, K: PC-3, L: U87-MG

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PCNA Antibody (339) [NB100-478] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 12% SDS PAGE diluted at 1:1000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PCNA Antibody (339) [NB100-478] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 12% SDS PAGE diluted at 1:1000